Protein_Domain
CALB

Part:BBa_K2302006:Design

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)


CALB,an enzyme that can hydrolyze fat to fatty acids.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 670
    Illegal NgoMIV site found at 780
    Illegal AgeI site found at 472
    Illegal AgeI site found at 730
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the codon usage is based on the biased codons of E. coli. We added additional amino acid residues (a functional signal peptide) at the N-termini of the CALB; the encoded recombinant CALB protein is therefore not the genuine one in terms of its primary sequence.


Source

it is a synthetic coding sequence.

References

Ayana Ujiie,Hideo Nakanoand Yugo Iwasaki.Extracellular production of Pseudozyma (Candida)antarctica lipase B with genuine primary sequence in recombinant Escherichia coli.J Biosci Bioeng,[J],2016,Mar;121(3):303–309.